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Neural Explant Cultures from Xenopus laevis
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JoVE Journal Neurociência
Neural Explant Cultures from Xenopus laevis

Neural Explant Cultures from Xenopus laevis

DOI:
10.3791/4232-v

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10:05 min

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October 15, 2012

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, , ,
1Department of Cell Biology,Harvard Medical School

Capítulos

  • 00:05Título
  • 00:57Introduction
  • 01:14In vitro Fertilization of Xenopus Eggs
  • 02:34Microinjection of RNA
  • 04:46Neural Tube Dissection and Plating
  • 09:08Results: Imaging Growth Cones from Xenopus Neuronal Explants
  • 09:40Conclusion

Summary

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Culturing neural explants from dissected Xenopus laevis embryos that express fluorescent fusion proteins allows for imaging of growth cone cytoskeletal dynamics.

Tags

Neural Explant CulturesXenopus LaevisAxon GuidanceGrowth ConeGuidance CuesCytoskeleton ModulationCytoskeletal Protein DynamicsEffector DynamicsHigh-resolution Live ImagingDevelopmental Neurobiology StudiesGrowth Cone Microtubule DynamicsFluorescently Tagged Cytoskeletal Fusion ProteinsGene Expression ManipulationNeural Tube StageOutgrowing Neurites

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