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ライブセル蛍光顕微鏡を用いた細菌毒素誘導される応答の可視化
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy
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ライブセル蛍光顕微鏡を用いた細菌毒素誘導される応答の可視化

11,595 Views

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14:29 min

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October 01, 2012

DOI:

10.3791/4227-v

DOI:
10.3791/4227-v

•

14:29 min
October 01, 2012

•

7 Views
, , ,
1Department of Immunology,University of Pittsburgh School of Medicine, 2Department of Cell Biology and Physiology,University of Pittsburgh School of Medicine

Tags

VisualizationBacterial ToxinLive Cell Fluorescence MicroscopyMembranePoresLeakageCellular ContentsInfluxToxin ExposureCell TypeInflammatory ResponsesImmune CellsMacrophagesPro-inflammatory CytokinesGram Positive BacteriaVirulence MechanismsMorphologic ChangesPlasma MembraneCholesterol-enriched Surface ProtrusionsExtracellular SpaceCellular Defense MechanismEM (electron Microscopy)Chemical FixationImmune CellsMacrophagesInflammationMembrane VesiclesIL-1 Family Cytokines

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Keyel, P. A., Heid, M. E., Watkins, S. C., Salter, R. D. Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy. J. Vis. Exp. (68), e4227, doi:10.3791/4227 (2012).

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