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Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy

Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy

11,615 Views

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14:29 min

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October 01, 2012

DOI:

10.3791/4227-v

DOI:
10.3791/4227-v

•

14:29 min
October 01, 2012

•

2 Views
, , ,
1Department of Immunology,University of Pittsburgh School of Medicine, 2Department of Cell Biology and Physiology,University of Pittsburgh School of Medicine

Summary

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Methods for purifying the cholesterol binding toxin streptolysin O from recombinant E. coli and visualization of toxin binding to live eukaryotic cells are described. Localized delivery of toxin induces rapid and complex changes in targeted cells revealing novel aspects of toxin biology.

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Keyel, P. A., Heid, M. E., Watkins, S. C., Salter, R. D. Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy. J. Vis. Exp. (68), e4227, doi:10.3791/4227 (2012).

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