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Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation

Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation

DOI:
10.3791/4208-v

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14:43 min

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October 01, 2012

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, , , ,
1Center for Inflammatory Bowel Diseases, Division of Digestive Diseases, David Geffen School of Medicine,The University of California Los Angeles, Los Angeles

Capítulos

  • 00:05Título
  • 01:26Experimental Setup
  • 02:39Donor Bone Marrow Extraction
  • 05:49Infusion to Recipient Mice
  • 07:19Induction of Colitis and Evaluation of Colitis
  • 08:55Quality Inspection of Bone Marrow Transplantation by Flow Cytometry
  • 11:59Transplantation Results
  • 14:21Conclusion

Summary

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Bone marrow transplantation provides a way to change the genotype of the bone marrow derived cells. If the gene of interest is expressed in both bone marrow derived cells and non-bone marrow derived cells, bone marrow transplantation can change the bone marrow derived cells to a different genotype without changing the non-bone marrow derived cell genotype.

Tags

Gene ExpressionImmune CellsNon-immune CellsMouse ColitisBone Marrow TransplantationGene-of-interestKnockout MiceGenotypeIrradiationDonor Bone MarrowRecipient MiceWild-type MiceCD Molecules (CD45.1 And CD45.2)

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