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Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria
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September 07, 2012
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Multi-parameter MeasurementPermeability Transition PoreIsolated Mouse Heart MitochondriaMitochondrial MembraneSolutesMolecular MassMtPTP FormationCyclophilin DVDACANTCell DeathMitochondrial Ca2+ HomeostasisBioenergeticsRedox SignalingMatrix Ca2+Oxidative StressAdenine Nucleotide DepletionHigh Concentrations Of PiMitochondrial Membrane DepolarizationUncouplingLong Chain Fatty AcidsIn VitroCalcium Retention CapacityProton Motive ForceMembrane Potential CollapseMitochondrial Matrix VolumeSwellingOuter Mitochondrial MembraneOrganelle FunctionFluorometric Measurement
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Marcu, R., Neeley, C. K., Karamanlidis, G., Hawkins, B. J. Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria. J. Vis. Exp. (67), e4131, doi:10.3791/4131 (2012).
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