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Hög genomströmning Rening av Affinity-märkta rekombinanta proteiner
JoVE Journal
Biologia
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JoVE Journal Biologia
High-throughput Purification of Affinity-tagged Recombinant Proteins
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Hög genomströmning Rening av Affinity-märkta rekombinanta proteiner

13,811 Views

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07:44 min

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August 26, 2012

DOI:

10.3791/4110-v

DOI:
10.3791/4110-v

•

07:44 min
August 26, 2012

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1 Views
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1Department of Life Sciences,Imperial College London

Tags

High-throughput PurificationAffinity-tagged Recombinant ProteinsX-ray CrystallographyBiochemical AnalysesStructure/function RelationshipsLarge-scale Mutagenesis StrategiesAmino Acids SubstitutionGain/loss-of-function PhenotypesCatalytic ActivityProtein StabilityProtein-protein Interaction SpecificityControlled And Reproducible MannerHigh-throughput StrategiesRobotic Liquid-handling PlatformsHis-tagged Recombinant ProteinsBasal Transcription Machinery

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Cite this Article

Wiesler, S. C., Weinzierl, R. O. High-throughput Purification of Affinity-tagged Recombinant Proteins. J. Vis. Exp. (66), e4110, doi:10.3791/4110 (2012).

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