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선호도 태그 재조합 단백질의 높은 처리량 정화

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

High-throughput Purification of Affinity-tagged Recombinant Proteins

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선호도 태그 재조합 단백질의 높은 처리량 정화

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07:44 min

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August 26, 2012

DOI:

DOI:

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07:44 min

August 26, 2012

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Simone C. Wiesler, Robert O.J. Weinzierl

¹Department of Life Sciences,Imperial College London

Tags

High-throughput Purification Affinity-tagged Recombinant Proteins X-ray Crystallography Biochemical Analyses Structure/function Relationships Large-scale Mutagenesis Strategies Amino Acids Substitution Gain/loss-of-function Phenotypes Catalytic Activity Protein Stability Protein-protein Interaction Specificity Controlled And Reproducible Manner High-throughput Strategies Robotic Liquid-handling Platforms His-tagged Recombinant Proteins Basal Transcription Machinery

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