/

/

आत्मीयता टैग पुनः संयोजक प्रोटीन के शोधन उच्च throughput

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

High-throughput Purification of Affinity-tagged Recombinant Proteins

Please note that all translations are automatically generated. Click here for the English version.

आत्मीयता टैग पुनः संयोजक प्रोटीन के शोधन उच्च throughput

13,811 Views

•

07:44 min

•

August 26, 2012

DOI:

DOI:

•

07:44 min

August 26, 2012

•

1 Views

Simone C. Wiesler, Robert O.J. Weinzierl

¹Department of Life Sciences,Imperial College London

Tags

High-throughput Purification Affinity-tagged Recombinant Proteins X-ray Crystallography Biochemical Analyses Structure/function Relationships Large-scale Mutagenesis Strategies Amino Acids Substitution Gain/loss-of-function Phenotypes Catalytic Activity Protein Stability Protein-protein Interaction Specificity Controlled And Reproducible Manner High-throughput Strategies Robotic Liquid-handling Platforms His-tagged Recombinant Proteins Basal Transcription Machinery

Read Article