This content is Free Access.
High-throughput Purificazione di affinità-tagged proteine ricombinanti
13,847 Views
•
07:44 min
•
August 26, 2012
DOI:
Summary
Automatically generated
Si descrive un metodo per la purificazione per affinità-tag di proteine ricombinanti utilizzando liquido movimentazione robotica. Questo metodo è generalmente applicabile alla piccola scala purificazione di solubili His-tagged proteine in una high-throughput formato.
Explore More Videos
High-throughput PurificationAffinity-tagged Recombinant ProteinsX-ray CrystallographyBiochemical AnalysesStructure/function RelationshipsLarge-scale Mutagenesis StrategiesAmino Acids SubstitutionGain/loss-of-function PhenotypesCatalytic ActivityProtein StabilityProtein-protein Interaction SpecificityControlled And Reproducible MannerHigh-throughput StrategiesRobotic Liquid-handling PlatformsHis-tagged Recombinant ProteinsBasal Transcription Machinery
Read Article
Cite this Article
Wiesler, S. C., Weinzierl, R. O. High-throughput Purification of Affinity-tagged Recombinant Proteins. J. Vis. Exp. (66), e4110, doi:10.3791/4110 (2012).
Copy