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高通量的亲和标签的重组蛋白的纯化

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

High-throughput Purification of Affinity-tagged Recombinant Proteins

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高通量的亲和标签的重组蛋白的纯化

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07:44 min

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August 26, 2012

DOI:

DOI:

•

07:44 min

August 26, 2012

•

5 Views

Simone C. Wiesler¹, Robert O.J. Weinzierl¹

¹Department of Life Sciences,Imperial College London

Summary

我们描述了一种方法，亲和标签的重组蛋白的纯化，使用的液体处理机器人。此方法通常是适用于小规模纯化的可溶性的带有His标记的蛋白质在高吞吐量的格式。

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