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Imunologia e Infecção

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分离人脐静脉内皮细胞和水流条件下其使用中的中性粒细胞轮回研究

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions

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分离人脐静脉内皮细胞和水流条件下其使用中的中性粒细胞轮回研究

DOI:

•

08:54 min

•

August 08, 2012

•

Anutosh Ganguly, Hong Zhang, Ritu Sharma, Sean Parsons, Kamala D. Patel

¹Department of Physiology and Pharmacology,University of Calgary

Capítulos

00:05Título
01:41Isolation and Maintenance of Human Vascular Endothelial Cells
04:54Neutrophil Recruitment and Transmigration
06:36Results: Fluorescent and Cell Migration Analysis of Neutrophil Transmigration Across Endothelial Cells in an Ibidi Chamber
08:03Conclusion

Summary

Tadução automática

本文首先介绍了人类脐静脉内皮细胞的分离的过程，然后演示如何使用这些细胞检查流量条件下的中性粒细胞轮回。通过使用低体积流量室从玻璃的光学特性的聚合物制成，活细胞荧光成像罕见的细胞群也是可能的。

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