Journal
/
Biologia
/
Generering af mus afledt fra induceret pluripotente stamceller
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Generation of Mice Derived from Induced Pluripotent Stem Cells

Generering af mus afledt fra induceret pluripotente stamceller

DOI:
10.3791/4003-v

•

11:56 min

•

November 29, 2012

•

, , , , , ,
1Dorris Neuroscience Center & Department of Cell Biology,The Scripps Research Institute , 2Mouse Genetics Core Facility,The Scripps Research Institute

Capítulos

  • 00:05Título
  • 02:12Preparation of Lentivirus
  • 03:24Derivation of iPSC Lines
  • 04:37Generation of Tetraploid Blastocysts
  • 07:20Preparation of iPSCs for Blastocyst Injection
  • 09:20Results: Reprogramming MEFs to iPSCs
  • 10:55Conclusion

Summary

Tadução automática

Tadução automática

Generering induceret pluripotente stamceller (IPSC) linjer producerer linjer af forskellig udviklingspotentiale, selv når de passerer standardtests for pluripotency. Her beskriver vi en protokol til at producere mus der udelukkende kommer fra iPSCs, der definerer de IPSC linjer som besidder fuld pluripotency<sup> 1</sup>.

Tags

Induced Pluripotent Stem CellsIPSCsBasic ResearchRegenerative TherapiesSomatic CellsGerm LayersEmbryoid BodiesTeratomasGene ExpressionChimeric MicePluripotency MarkersDonor Cell SourcesSelection MethodsTetraploid Embryo ComplementationFull PluripotencyElectrofusionBlastocyst Stage

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System

Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set

Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells

Selektion og isolering Kolonier af Human induceret pluripotente stamceller omprogrammeret fra voksne fibroblaster

Omprogrammering humane somatiske celler i induceret pluripotente stamceller (IPSC'er) under anvendelse af retroviral vektor med GFP

Generation of Human induceret pluripotente stamceller fra perifert blod Brug af STEMCCA lentivirusvektor

Hurtig og effektiv generering af neuroner fra humane pluripotente stamceller i en Multititre Plate Format

Generation of Human cardiomyocytes: En Differentiering Protocol fra Feeder-fri Menneskelige induceret pluripotente stamceller

Effektiv Generation Menneskelige induceret pluripotente stamceller fra humane somatiske celler med Sendai-virus

Undertype-specifikke optisk aktionspotentialet optagelser i menneskelige induceret pluripotente stamceller-afledt ventrikulær Cardiomyocytes

Read Article