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诱导多能干细胞来源于小鼠的生成

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Generation of Mice Derived from Induced Pluripotent Stem Cells

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诱导多能干细胞来源于小鼠的生成

DOI:

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11:56 min

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November 29, 2012

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Michael J. Boland, Jennifer L. Hazen, Kristopher L. Nazor, Alberto R. Rodriguez, Greg Martin, Sergey Kupriyanov, Kristin K. Baldwin

¹Dorris Neuroscience Center & Department of Cell Biology,The Scripps Research Institute , ²Mouse Genetics Core Facility,The Scripps Research Institute

Capítulos

00:05Título
02:12Preparation of Lentivirus
03:24Derivation of iPSC Lines
04:37Generation of Tetraploid Blastocysts
07:20Preparation of iPSCs for Blastocyst Injection
09:20Results: Reprogramming MEFs to iPSCs
10:55Conclusion

Summary

Tadução automática

生成诱导多能干细胞（IPSC）线生产线，不同的发展潜力，即使当他们通过标准测试的多能性。在这里，我们描述了一个协议，全部来自iPS细胞拥有完整的多能性，它定义了IPSC的小鼠<sup> 1</sup>。

Tags

Induced Pluripotent Stem Cells IPSCs Basic Research Regenerative Therapies Somatic Cells Germ Layers Embryoid Bodies Teratomas Gene Expression Chimeric Mice Pluripotency Markers Donor Cell Sources Selection Methods Tetraploid Embryo Complementation Full Pluripotency Electrofusion Blastocyst Stage

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