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Capítulos
- 00:00Título
- 01:35Crystallisation Plate Preparation
- 03:54Robot I: In Meso Robot
- 08:48Priming the Syringe
- 09:27Running the Robot
- 11:14Sealing the Plate
- 13:13Robot 2: Mosquito LCP
- 18:00Robot 3: Gryphon LCP
- 19:55Concluding Remarks
Heri beskrives en robot tilgang til high-throughput krystallisation af membranproteiner i lipidiske mesofaser til anvendelse i strukturbestemmelse ved hjælp af makromolekylære røntgenkrystallografi. Tre robotter kan håndtere den viskose og klæbende protein-ladede mesofase integreret med fremgangsmåden introduceres.
Tags
RobotHigh-throughput CrystallizationMembrane ProteinsLipidic MesophasesMacromolecular X-ray CrystallographyMXDiffraction-quality CrystalsIn Meso MethodLipidic Cubic Phase (LCP) MethodBicontinuous MesophaseToothpaste-like ConsistencyDispensingReproducible MannerSmall VolumesCrystallization WellsManual ApproachSpecialty ApplicationsCrystal OptimizationDerivatization
