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神经元的初步培养体系的研究疱疹病毒潜伏期和活化
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation
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神经元的初步培养体系的研究疱疹病毒潜伏期和活化

17,119 Views

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12:22 min

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April 02, 2012

DOI:

10.3791/3823-v

DOI:
10.3791/3823-v

•

12:22 min
April 02, 2012

•

1 Views
, , , , , ,
1Department of Microbiology,New York University School of Medicine, 2Molecular Neurobiology Program, Skirball Institute for Biomolecular Medicine,New York University School of Medicine, 3Department of Otolaryngology,New York University School of Medicine, 4Department of Cell Biology,New York University School of Medicine, 5Department of Physiology and Neuroscience,New York University School of Medicine, 6Department of Psychiatry,New York University School of Medicine, 7Center for Neural Science,New York University School of Medicine

Summary

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协议描述了一种高效和可重复性的模型系统研究单纯疱疹病毒1型（HSV-1）延迟和重新启动。该法采用同质交感神经神经元文化，并允许使用各种工具，包括RNA干扰和重组蛋白表达的病毒神经元相互作用的分子解剖。

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Cite this Article

Kobayashi, M., Kim, J., Camarena, V., Roehm, P. C., Chao, M. V., Wilson, A. C., Mohr, I. A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation. J. Vis. Exp. (62), e3823, doi:10.3791/3823 (2012).

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