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A Genetic Screen to Isolate Toxoplasma gondii Host-cell Egress Mutants
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
A Genetic Screen to Isolate Toxoplasma gondii Host-cell Egress Mutants

A Genetic Screen to Isolate Toxoplasma gondii Host-cell Egress Mutants

DOI:
10.3791/3807-v

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14:56 min

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February 08, 2012

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1Department of Biology,Boston College

Capítulos

  • 00:05Título
  • 01:39Titration of Mutagen
  • 05:59Enrichment of Egress Mutants
  • 08:48Validation of Mutant Phenotypes by Egress Assays
  • 10:21Cloning of Egress Mutants by Limiting Dilution
  • 12:41Representative Data
  • 14:14Conclusion

Summary

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Forward genetics is a powerful method to unravel the molecular level of how Toxoplasma egresses from its host cell. Protocols are provided to chemically mutagenize parasites, enrich for mutants with defects in induced egress, and validate the phenotype of cloned mutants.

Tags

Genetic ScreenToxoplasma GondiiHost-cell Egress MutantsObligate Intracellular ParasiteLytic Replication CycleActive InvasionReplication Inside Host CellActive EgressMolecular LevelSignaling PathwaysPharmacological AgentsTriggers Of EgressIntracellular Ca2+Candidate Gene ApproachPlant Like Calcium Dependent Protein Kinase (CDPK)(chemical) Genetic ApproachesScreen For Mutant ParasitesDefect In Host Cell EgressChemical MutagenesisN-ethyl-N-nitrosourea (ENU)

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