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Imunologia e Infecção

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斑马鱼的胚胎细胞内细菌病原体感染

JoVE Journal
Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens

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斑马鱼的胚胎细胞内细菌病原体感染

DOI:

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11:18 min

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March 15, 2012

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Erica L. Benard, Astrid M. van der Sar, Felix Ellett, Graham J. Lieschke, Herman P. Spaink, Annemarie H. Meijer

¹Department of Molecular Cell Biology, Institute of Biology,Leiden University, ²Department of Medical Microbiology and Infection Control,VU University Medical Center, ³Australian Regenerative Medicine Institute,Monash University

Capítulos

00:05Título
01:42Intravenous Injection of Bacteria into One-day Old Embryos
05:00Alternate Routes of Infection
06:52Stereo and Confocal Imaging of the Infection
08:07Representative Pathogenic Infection Results
10:43Conclusion

Summary

Tadução automática

透明斑马鱼的胚胎已经证明有用的模型可视化和主机之间的先天免疫细胞和细胞内细菌病原体的功能，如研究的相互作用，<em>鼠伤寒沙门氏菌</em>和<em>分枝杆菌</em>。微注射细菌和多色荧光成像是在斑马鱼胚胎感染模型中的应用，所涉及的基本技术。

Tags

Zebrafish Embryos Intracellular Bacterial Pathogens Host-pathogen Interactions Innate Immune System Macrophages Neutrophils Lymphocytes Adaptive Immune Responses Genetic Tools Mutant Resources Transgenic Reporter Lines Salmonella Enterica Serovar Typhimurium Mycobacterium Marinum Infection Processes Acute Infection Chronic Infection Micro-injection Blood Circulation

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