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Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens
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Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens
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March 15, 2012
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Transparent zebrafish embryos have proved useful model hosts to visualize and functionally study interactions between innate immune cells and intracellular bacterial pathogens, such as Salmonella typhimurium and Mycobacterium marinum. Micro-injection of bacteria and multi-color fluorescence imaging are essential techniques involved in the application of zebrafish embryo infection models.
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Zebrafish EmbryosIntracellular Bacterial PathogensHost-pathogen InteractionsInnate Immune SystemMacrophagesNeutrophilsLymphocytesAdaptive Immune ResponsesGenetic ToolsMutant ResourcesTransgenic Reporter LinesSalmonella Enterica Serovar TyphimuriumMycobacterium MarinumInfection ProcessesAcute InfectionChronic InfectionMicro-injectionBlood Circulation
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Benard, E. L., van der Sar, A. M., Ellett, F., Lieschke, G. J., Spaink, H. P., Meijer, A. H. Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens. J. Vis. Exp. (61), e3781, doi:10.3791/3781 (2012).
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