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Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens
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Capítulos
- 00:05Título
- 01:42Intravenous Injection of Bacteria into One-day Old Embryos
- 05:00Alternate Routes of Infection
- 06:52Stereo and Confocal Imaging of the Infection
- 08:07Representative Pathogenic Infection Results
- 10:43Conclusion
Transparent zebrafish embryos have proved useful model hosts to visualize and functionally study interactions between innate immune cells and intracellular bacterial pathogens, such as Salmonella typhimurium and Mycobacterium marinum. Micro-injection of bacteria and multi-color fluorescence imaging are essential techniques involved in the application of zebrafish embryo infection models.
Tags
Zebrafish EmbryosIntracellular Bacterial PathogensHost-pathogen InteractionsInnate Immune SystemMacrophagesNeutrophilsLymphocytesAdaptive Immune ResponsesGenetic ToolsMutant ResourcesTransgenic Reporter LinesSalmonella Enterica Serovar TyphimuriumMycobacterium MarinumInfection ProcessesAcute InfectionChronic InfectionMicro-injectionBlood Circulation
