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Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue
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Biologia
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JoVE Journal Biologia
Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue

15,326 Views

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17:38 min

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April 27, 2012

DOI:

10.3791/3764-v

DOI:
10.3791/3764-v

•

17:38 min
April 27, 2012

•

1 Views
, ,
1Howard Hughes Medical Institute, Department of Neurobiology,Duke University , 2Department of Biological Sciences,Hokkaido University

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Radioactive In Situ HybridizationGene Expression PatternsTimingLevelCellular LocalizationCell TypeMRNAComplementary RNA ProbesRadioactive NucleotidesDetection SensitivityQuantitative AnalysisLight Sensitive X-ray FilmEmulsion CoatedTarget Gene ExpressionNon-radioactive Probe MethodsDIG-labelingAmplification StepsHRP-antibodiesTSA KitSignal IntensityTargeted MRNA AmountsQuantitative AnalysisDevelopmental Stages Of Embryos

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Chen, C., Wada, K., Jarvis, E. D. Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue. J. Vis. Exp. (62), e3764, doi:10.3791/3764 (2012).

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