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Genöverföring till utvecklingsländerna Mouse innerörat genom<em> In Vivo</em> Elektroporering
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Capítulos
- 00:05Título
- 01:58Part 1: Ventral Laparotomy
- 05:23Ventral Midline Incision
- 07:46Part 2: Transuterine Microinjection
- 08:32Common Embryo Orientations Observed by Transillumination of the Uterus
- 10:19Embryo Reorientation for Otocyst Injections
- 13:48Transuterine Microinjection
- 16:31Part 3: In vivo Electroporation
- 17:37Postnatal Selection of a Pup whose Otocyst was Injected at E12.5
- 19:47Prophylactic Analgesia
- 20:18Surgical Subject the Morning after Surgery
- 20:55Part 4: Representative Results
Musen innerörat är en placode-derived sinnesorgan, vars utvecklande program utarbetas under dräktigheten. Vi definierar en<em> I livmodern</em> Genöverföring tekniken består av tre steg: mus ventrala laparotomi, transuterine mikroinjektion och<em> In vivo</em> Elektroporering. Vi använder digital video mikroskopi för att påvisa de kritiska experimentella embryologiska tekniker.
Tags
Gene TransferDeveloping Mouse Inner EarIn Vivo ElectroporationSensory EpitheliaCristaeMaculaeOrgan Of CortiVestibular Hair CellsAuditory Hair CellsCell Fate SpecificationMorphogenesisGestationTransgenic EmbryosMolecular BasisGain-of-function StudiesLoss-of-function StudiesGene Misexpression StudiesVentral LaparotomyTransuterine Microinjection
