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Gentransfer an sich entwickelnden Maus Innenohr<em> In Vivo</em> Die Elektroporation
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Capítulos
- 00:05Título
- 01:58Part 1: Ventral Laparotomy
- 05:23Ventral Midline Incision
- 07:46Part 2: Transuterine Microinjection
- 08:32Common Embryo Orientations Observed by Transillumination of the Uterus
- 10:19Embryo Reorientation for Otocyst Injections
- 13:48Transuterine Microinjection
- 16:31Part 3: In vivo Electroporation
- 17:37Postnatal Selection of a Pup whose Otocyst was Injected at E12.5
- 19:47Prophylactic Analgesia
- 20:18Surgical Subject the Morning after Surgery
- 20:55Part 4: Representative Results
Die Maus ist eine Innenohr-derived Plakode Sinnesorgan, dessen Entwicklungs-Programm wird während der Schwangerschaft erarbeitet. Wir definieren ein<em> In der Gebärmutter</em> Gentransfer-Technik, bestehend aus drei Schritten: Maus ventralen Laparotomie, transuterine Mikroinjektion, und<em> In-vivo-</em> Elektroporation. Wir nutzen digitale Video-Mikroskopie, um die kritischen experimentellen embryologischen Techniken zu demonstrieren.
Tags
Gene TransferDeveloping Mouse Inner EarIn Vivo ElectroporationSensory EpitheliaCristaeMaculaeOrgan Of CortiVestibular Hair CellsAuditory Hair CellsCell Fate SpecificationMorphogenesisGestationTransgenic EmbryosMolecular BasisGain-of-function StudiesLoss-of-function StudiesGene Misexpression StudiesVentral LaparotomyTransuterine Microinjection
