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Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation
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Biologia
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JoVE Journal Biologia
Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation

Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation

17,603 Views

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07:15 min

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November 26, 2011

DOI:

10.3791/3602-v

DOI:
10.3791/3602-v

•

07:15 min
November 26, 2011

•

6 Views
,
1Department of Chemistry,Tunghai University, 2Department of Biochemistry,University of California, Riverside

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MALDI-TOF mass spectrometry was successfully utilized to monitor the amide hydrogen/deuterium exchange in protein kinase Pak2 activation.

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Amide Hydrogen/deuterium ExchangeMALDI-TOF Mass SpectrometryProtein-protein InteractionsProtein Conformational ChangesProtein DynamicsProtein-ligand InteractionsDeuteration RatesPepsin DigestionResolutionHeteronuclear Single Quantum Coherence (HSQC)NMRAqueous SolutionMALDI-TOF DetectionHPLC/ESI-MS SystemProtein Kinase Pak2Gamma-Pak

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Cite this Article

Hsu, Y., Traugh, J. A. Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation. J. Vis. Exp. (57), e3602, doi:10.3791/3602 (2011).

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