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Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System
 

Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System

Article DOI: 10.3791/3597-v • 09:39 min • April 15th, 2012
April 15th, 2012 •
1, 2, 1, 2, 1
1Department of Biological Sciences, Florida Atlantic University, 2Department of Chemistry & Biochemistry, Florida Atlantic University

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A rapid in vivo assay to test for neuromodulatory compounds using the Giant Fiber System (GFS) of Drosophila melanogaster is described. Nanoinjections in the head of the animal along with electrophysiological recordings of the GFS can reveal bioactivity of compounds on neurons or muscles.

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Nanoinjection Electrophysiology Assay Screen Bioactivity Compounds Drosophila Melanogaster Giant Fiber System In Vivo Activity Pharmacological Agents Modulatory Effects Neuronal Circuit Small Molecules Peptides Molecular Targets Gap Junctions Cholinergic Nicotinic Acetylcholine Receptors Neuromuscular Junctions Glutamatergic Nanoliter Quantities Intracellular Recordings
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