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Determining the Phagocytic Activity of Clinical Antibody Samples
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November 30, 2011
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We present a high-throughput flow cytometric assay to determine the phagocytic activity of antigen-specific antibodies from clinical samples, utilizing fluorescent antigen-coated beads and a monocytic cell line expressing multiple Fc receptors—providing receptor usage and phagocytic activity determinations in a standardized and reproducible fashion for any antigen of interest.
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McAndrew, E. G., Dugast, A., Licht, A. F., Eusebio, J. R., Alter, G., Ackerman, M. E. Determining the Phagocytic Activity of Clinical Antibody Samples. J. Vis. Exp. (57), e3588, doi:10.3791/3588 (2011).
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