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Imunologia e Infecção
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Intravital Imaging of the Mouse Thymus mittels 2-Photonen-Mikroskopie
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy
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Intravital Imaging of the Mouse Thymus mittels 2-Photonen-Mikroskopie

DOI:
10.3791/3504-v

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10:08 min

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January 07, 2012

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, ,
1Laboratory of Immune Regulation,Instituto Gulbenkian de Ciência

Capítulos

  • 00:01Título
  • 00:05Introduction
  • 01:15Animal preparation: Irradiation and bone marrow transfer
  • 03:16Animal preparation: Thymic transplantation
  • 04:58Animal preparation: Surgery to exposure transplanted thymus
  • 06:53Two-photon image acquisition
  • 08:35Resultados

Summary

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We have developed novel laboratory tools and protocols for intravital imaging acquisition of the thymus. Our technique should help in the identification of “niches” within the thymus where T cell development occurs.

Tags

Intravital ImagingMouse Thymus2-photon MicroscopyImage AcquisitionOrgansStereotactic ToolsSurgical ProceduresCellular BehaviorsLive AnimalsEx Vivo Imaging AcquisitionSurgical ProcedureOrgan HolderFoxp3+ CellsRegulatory T CellsNaturally-occurring Tregs (nTregs)Peripherally-converted Tregs (pTregs)In Vivo InteractionsPhenotype And PhysiologyNude Mice

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