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活体成像的鼠标使用2 - 光子显微镜的胸腺

JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção

Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy

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活体成像的鼠标使用2 - 光子显微镜的胸腺

DOI:

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10:08 min

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January 07, 2012

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Susana S. Caetano, Tatiana Teixeira, Carlos E. Tadokoro

¹Laboratory of Immune Regulation,Instituto Gulbenkian de Ciência

Capítulos

00:01Título
00:05Introduction
01:15Animal preparation: Irradiation and bone marrow transfer
03:16Animal preparation: Thymic transplantation
04:58Animal preparation: Surgery to exposure transplanted thymus
06:53Two-photon image acquisition
08:35Resultados

Summary

Tadução automática

我们已经开发出新的实验室工具和胸腺的活体成像收购协议。我们的技术帮助识别“龛”胸腺内T细胞发育时。

Tags

Intravital Imaging Mouse Thymus 2-photon Microscopy Image Acquisition Organs Stereotactic Tools Surgical Procedures Cellular Behaviors Live Animals Ex Vivo Imaging Acquisition Surgical Procedure Organ Holder Foxp3+ Cells Regulatory T Cells Naturally-occurring Tregs (nTregs)Peripherally-converted Tregs (pTregs)In Vivo Interactions Phenotype And Physiology Nude Mice

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