Journal
/
Neurociência
/
鼠标感觉神经支配的皮肤和四肢骨骼纤维组织的制备及免疫染色
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Tissue Preparation and Immunostaining of Mouse Sensory Nerve Fibers Innervating Skin and Limb Bones
Please note that all translations are automatically generated. Click here for the English version.

鼠标感觉神经支配的皮肤和四肢骨骼纤维组织的制备及免疫染色

DOI:
10.3791/3485-v

•

10:25 min

•

January 26, 2012

•

,
1Department of Pharmacology,The University of Iowa, 2Department of Anesthesia,Roy J. and Lucille A. Carver College of Medicine, The University of Iowa

Capítulos

  • 00:05Título
  • 01:42Tissue Dissection, Post-fixation, and Sectioning
  • 05:37Immunostaining: Floating Section Staining for Plantar Punch Tissue
  • 07:17Immunostaining: On-slide Staining for Limb Bone Sections
  • 08:38Epifluorescence and Confocal Imaging of Immunostained Plantar Punch and Bone Tissue

Summary

Tadução automática

Tadução automática

末梢感觉神经纤维亚型的免疫细胞化学鉴定（和蛋白表达其中的检测）底层外围感觉的分子机制的理解是关键。在这里，我们描述外设/内脏组织样本，特定的免疫外周感觉神经纤维，如皮肤和四肢骨骼的制备方法。

Tags

ImmunostainingMouse Sensory Nerve FibersSkin InnervationLimb Bones InnervationSensory PerceptionReceptorsIon Channel ProteinsElectrophysiological MethodsVisceral Organs InnervationBone InnervationNon-excitable ProteinsImmunostaining ProceduresSensory GangliaProtein Expression Studies

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

制备小鼠脑组织中的免疫电镜

成像遥感信息素在小鼠犁鼻急性组织切片的制备

禽流感胚胎听神经听觉神经纤维的选择性跟踪

一个孤立的半完整的上下游鼠标前庭上皮细胞电生理和高分辨率的双光子显微镜

在皮肤中的单神经末梢体内双光子显微镜

小鼠皮肤及其毛囊图案和感觉轴突形态分析中的应用平山成像

小鼠胚胎感觉轴突预测整个安装成像

人 Intraepidermal 神经纤维内线粒体三维成像及分析

一种方法为Target和隔离气道支配感觉神经元的小鼠

从美国牛蛙的小囊（毛细胞的生理准备<em>牛蛙</em>）

Read Article