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Electrophysiological Characterization of GFP-Expressing Cell Populations in the Intact Retina
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Electrophysiological Characterization of GFP-Expressing Cell Populations in the Intact Retina
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November 14, 2011
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This article depicts the recording of individual cells from fluorescently tagged neuronal populations in the intact mouse retina. By using two-photon infrared excitation transgenetically labeled cells were targeted for patch-clamp recording to study their light responses, receptive field properties, and morphology.
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Electrophysiological CharacterizationGFP-expressing Cell PopulationsIntact RetinaPhysiological PropertiesSynaptic ConnectionsRetinal Amacrine CellsInterneuronsRecording ElectrodeTransgenic Mouse LinesFluorescent MarkersDirected Microelectrode TargetingPhototoxicityPhotoreceptorsPhotopigment BleachingLight-adapted Retinal Circuits
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Pottek, M., Knop, G. C., Weiler, R., Dedek, K. Electrophysiological Characterization of GFP-Expressing Cell Populations in the Intact Retina. J. Vis. Exp. (57), e3457, doi:10.3791/3457 (2011).
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