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Organotypische Slice culturen van embryonale ventrale middenhersenen: Een systeem om Dopaminerge Neuronale Development Study In vitro</em
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Neurociência
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JoVE Journal Neurociência
Organotypic Slice Cultures of Embryonic Ventral Midbrain: A System to Study Dopaminergic Neuronal Development in vitro
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Organotypische Slice culturen van embryonale ventrale middenhersenen: Een systeem om Dopaminerge Neuronale Development Study In vitro</em

DOI:
10.3791/3350-v

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07:33 min

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January 31, 2012

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,
1Institute of Reconstructive Neurobiology,University of Bonn

Capítulos

  • 00:05Título
  • 01:00Preparation for Dissection
  • 03:00Embryonic Brain Preparation and Vibratome Sectioning
  • 05:09Slice Culture and Time-lapse Imaging
  • 06:21Results: Dopaminergic Neuronal Development in Organotypic Slice Cultures of Embryonic Ventral Midbrain
  • 07:04Conclusion

Summary

Tadução automática

Tadução automática

Een methode om organotypische segmenten van de E12.5 muriene embryonale middenhersenen genereren is beschreven. De organotypische slice culturen kan worden gebruikt om het gedrag van dopaminerge neuronen of andere ventrale middenhersenen neuronen te observeren.

Tags

Organotypic Slice CulturesEmbryonic Ventral MidbrainDopaminergic Neuronal DevelopmentIn VitroMouse Model OrganismBrain DevelopmentMolecular DataGenetic DataOrganotypic Slice Cultures Of Embryonic BrainsMurine Brain DevelopmentEx-vivo ManipulationTransgenic MiceGene ExpressionFluorescent ProteinsTime-lapse ImagingCerebral Cortex DevelopmentForebrainVentral Brain AreasNeuronal ClustersMigratory TrajectoriesFinal Position

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