/
/
Organotypic Slice Cultures of Embryonic Ventral Midbrain: A System to Study Dopaminergic Neuronal Development in vitro
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Organotypic Slice Cultures of Embryonic Ventral Midbrain: A System to Study Dopaminergic Neuronal Development in vitro
17,736 Views
•
07:33 min
•
January 31, 2012
DOI:
Summary
Automatically generated
A method to generate organotypic slices from the E12.5 murine embryonic midbrain is described. The organotypic slice cultures can be used to observe the behavior of dopaminergic neurons or other ventral midbrain neurons.
Explore More Videos
Organotypic Slice CulturesEmbryonic Ventral MidbrainDopaminergic Neuronal DevelopmentIn VitroMouse Model OrganismBrain DevelopmentMolecular DataGenetic DataOrganotypic Slice Cultures Of Embryonic BrainsMurine Brain DevelopmentEx-vivo ManipulationTransgenic MiceGene ExpressionFluorescent ProteinsTime-lapse ImagingCerebral Cortex DevelopmentForebrainVentral Brain AreasNeuronal ClustersMigratory TrajectoriesFinal Position
Read Article
Cite this Article
Bodea, G. O., Blaess, S. Organotypic Slice Cultures of Embryonic Ventral Midbrain: A System to Study Dopaminergic Neuronal Development in vitro. J. Vis. Exp. (59), e3350, doi:10.3791/3350 (2012).
Copy