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胚胎腹侧中脑器官型片文化：一个系统来研究多巴胺能神经元发展在体外</em
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Neurociência
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JoVE Journal Neurociência
Organotypic Slice Cultures of Embryonic Ventral Midbrain: A System to Study Dopaminergic Neuronal Development in vitro
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胚胎腹侧中脑器官型片文化：一个系统来研究多巴胺能神经元发展在体外</em

DOI:
10.3791/3350-v

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07:33 min

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January 31, 2012

•

,
1Institute of Reconstructive Neurobiology,University of Bonn

Capítulos

  • 00:05Título
  • 01:00Preparation for Dissection
  • 03:00Embryonic Brain Preparation and Vibratome Sectioning
  • 05:09Slice Culture and Time-lapse Imaging
  • 06:21Results: Dopaminergic Neuronal Development in Organotypic Slice Cultures of Embryonic Ventral Midbrain
  • 07:04Conclusion

Summary

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描述一个方法来生成从E12.5小鼠胚胎脑器官切片。可用于器官切片文化观察多巴胺能神经元或其他腹侧中脑的神经元的行为。

Tags

Organotypic Slice CulturesEmbryonic Ventral MidbrainDopaminergic Neuronal DevelopmentIn VitroMouse Model OrganismBrain DevelopmentMolecular DataGenetic DataOrganotypic Slice Cultures Of Embryonic BrainsMurine Brain DevelopmentEx-vivo ManipulationTransgenic MiceGene ExpressionFluorescent ProteinsTime-lapse ImagingCerebral Cortex DevelopmentForebrainVentral Brain AreasNeuronal ClustersMigratory TrajectoriesFinal Position

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