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Patch-clamp Capacitance Measurements and Ca2+ Imaging at Single Nerve Terminals in Retinal Slices
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Neurociência
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JoVE Journal Neurociência
Patch-clamp Capacitance Measurements and Ca2+ Imaging at Single Nerve Terminals in Retinal Slices

Patch-clamp Capacitance Measurements and Ca2+ Imaging at Single Nerve Terminals in Retinal Slices

DOI:
10.3791/3345-v

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09:16 min

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January 19, 2012

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, ,
1The Vollum Institute,Oregon Health and Science University

Capítulos

  • 00:05Título
  • 01:37Preparation of Agar-embedded Retinal Slices
  • 04:21Electrophysiological Recordings and Ca2+ Imaging
  • 07:23Results: Identification and Ca2+ Imaging of Bipolar Cell Synaptic Terminals in Retinal Slices
  • 08:49Conclusion

Summary

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Tadução automática

Here we describe a protocol for the preparation of agar-embedded retinal slices that are suitable for electrophysiology and Ca2+ imaging. This method allows one to study ribbon-type synapses in retinal microcircuits using direct patch-clamp recordings of single presynaptic nerve terminals.

Tags

Patch-clamp Capacitance MeasurementsCa2+ ImagingSingle Nerve TerminalsRetinal SlicesVisual StimuliDynamic RangeLight IntensitiesFrequency ChangesSpecialized NeuronsVertebrate RetinaPhotoreceptorsBipolar CellsRibbon-type Active ZonesNeurotransmitter ReleaseON-type Mixed Bipolar Cell (Mb) TerminalsGoldfish RetinaRod And Cone Photoreceptor InputRibbon-type SynapsesSize Of TerminalsLateral And Reciprocal Synaptic ConnectionsAmacrine Cell DendritesPatch-clamp TechniquePresynaptic Ca2+ CurrentsMembrane Capacitance ChangesReciprocal Synaptic Feedback InhibitionGABAA ReceptorsGABAC ReceptorsExocytosisShort-term Plasticity Of Excitatory Neurotransmitter ReleaseInhibitory Neurotransmitter Release From Amacrine Cells

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