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Neuroscience

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JoVE Journal Neuroscience
Patch-clamp Capacitance Measurements and Ca2+ Imaging at Single Nerve Terminals in Retinal Slices
 

Patch-clamp Capacitance Measurements and Ca2+ Imaging at Single Nerve Terminals in Retinal Slices

Article DOI: 10.3791/3345-v • 09:17 min • January 19th, 2012
January 19th, 2012 •
1, 1, 1
1The Vollum Institute, Oregon Health and Science University

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résumé
Automatic Translation

Here we describe a protocol for the preparation of agar-embedded retinal slices that are suitable for electrophysiology and Ca2+ imaging. This method allows one to study ribbon-type synapses in retinal microcircuits using direct patch-clamp recordings of single presynaptic nerve terminals.

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Patch-clamp Capacitance Measurements Ca2+ Imaging Single Nerve Terminals Retinal Slices Visual Stimuli Dynamic Range Light Intensities Frequency Changes Specialized Neurons Vertebrate Retina Photoreceptors Bipolar Cells Ribbon-type Active Zones Neurotransmitter Release ON-type Mixed Bipolar Cell (Mb) Terminals Goldfish Retina Rod And Cone Photoreceptor Input Ribbon-type Synapses Size Of Terminals Lateral And Reciprocal Synaptic Connections Amacrine Cell Dendrites Patch-clamp Technique Presynaptic Ca2+ Currents Membrane Capacitance Changes Reciprocal Synaptic Feedback Inhibition GABAA Receptors GABAC Receptors Exocytosis Short-term Plasticity Of Excitatory Neurotransmitter Release Inhibitory Neurotransmitter Release From Amacrine Cells
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