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在复杂的临床和环境样本使用寡核苷酸加上荧光微多元检测细菌
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11:09 min
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October 23, 2011
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Summary
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我们描述了一个复用的微生物样本内使用寡核苷酸耦合的荧光珠的检测方法。从样本内的所有生物体的扩增杂交的探针耦合珠小组。一个Luminex公司或生物复杂的仪器是用于查询每个珠珠型和杂交信号。
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Dumonceaux, T. J., Town, J. R., Hill, J. E., Chaban, B. L., Hemmingsen, S. M. Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres. J. Vis. Exp. (56), e3344, doi:10.3791/3344 (2011).
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