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Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes
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Biologia
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JoVE Journal Biologia
Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

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08:23 min

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December 28, 2011

DOI:

10.3791/3302-v

DOI:
10.3791/3302-v

•

08:23 min
December 28, 2011

•

1 Views
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1Buck Institute for Research on Aging, 2Department of Physiology & Biophysics,University of Washington

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Single CellTranscriptional ProfilingAdult MouseCardiomyocytesGene ExpressionHomogenatesStochastic VariationPure Cardiomyocyte PopulationsCollagenase PerfusionMicroarraysWhole TranscriptomeQPCRNanofluidic ArraysGene Expression ProfilesEvaluation MetricsVariance Between CellsRobust AmplificationDouble Stranded CDNANimbleGen ArraysReverse Transcriptase – Specific Target Amplification (RT-STA)Nanofluidic PCRVariability Of Expression

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Flynn, J. M., Santana, L. F., Melov, S. Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes. J. Vis. Exp. (58), e3302, doi:10.3791/3302 (2011).

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