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Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes
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JoVE Journal Biology
Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

DOI:
10.3791/3302-v

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08:23 min

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December 28, 2011

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, ,
1Buck Institute for Research on Aging, 2Department of Physiology & Biophysics,University of Washington

Chapters

  • 00:05Title
  • 01:15Isolating Cardiomyocyte
  • 03:37Isolating Single Cells
  • 04:08Single Cell qPCR
  • 05:26Whole Transcriptome Amplification and Microarray of Single Cells
  • 07:00Quality Control Results
  • 07:56Conclusion

Summary

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Single cell expression profiling allows the detailed gene expression analysis of individual cells. We describe methods for the isolation of cardiomyocytes, and preparing the resulting lysates for either whole transcriptome microarray or qPCR of specific targets.

Tags

Single CellTranscriptional ProfilingAdult MouseCardiomyocytesGene ExpressionHomogenatesStochastic VariationPure Cardiomyocyte PopulationsCollagenase PerfusionMicroarraysWhole TranscriptomeQPCRNanofluidic ArraysGene Expression ProfilesEvaluation MetricsVariance Between CellsRobust AmplificationDouble Stranded CDNANimbleGen ArraysReverse Transcriptase – Specific Target Amplification (RT-STA)Nanofluidic PCRVariability Of Expression

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