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生体内ビデオ顕微鏡による組織内好中球腔内クロールを追跡、経内皮移動と走
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15:01 min
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September 24, 2011
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Summary
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我々は、in vivoでの好中球走化性因子のソースに対応して好中球動員中に動的な好中球 - 内皮細胞の相互作用を測定するための明生体顕微鏡検査のプロトコルを記述する。好中球内腔クロール、マウス精巣挙筋の組織の経内皮移動と走化性は、時間の経過したビデオ撮影すると、ImageJで追跡と視覚化されます。
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Xu, N., Lei, X., Liu, L. Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy. J. Vis. Exp. (55), e3296, doi:10.3791/3296 (2011).
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