/
/
Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy
19,464 Views
•
15:01 min
•
September 24, 2011
DOI:
Summary
Automatically generated
We describe a protocol of brightfield intravital microscopy for measuring dynamic neutrophil-endothelial cell interactions during neutrophil recruitment in response to the source of a neutrophil chemoattractant in vivo. Neutrophil intraluminal crawling, transendothelial migration and chemotaxis in mouse cremaster muscle tissue are visualized with time-lapsed video photography and tracked with ImageJ.
Explore More Videos
TrackingNeutrophilIntraluminal CrawlingTransendothelial MigrationChemotaxisIntravital Video MicroscopyLeukocytesInflammationPostcapillary VenulesLuminal SurfaceRolling LeukocytesEndotheliumFirm AdhesionChemokineChemoattractantsVenular SurfaceIntraluminal CrawlingTransmigrationChemoattractant GradientBrightfield Intravital MicroscopyMouse Cremaster MuscleNeutrophil RecruitmentAgarose Gel
Read Article
Cite this Article
Xu, N., Lei, X., Liu, L. Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy. J. Vis. Exp. (55), e3296, doi:10.3791/3296 (2011).
Copy