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Bilaminar Co-kultur Primary Rat Kortikal neuroner og glia
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Neurociência
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JoVE Journal Neurociência
Bilaminar Co-culture of Primary Rat Cortical Neurons and Glia

Bilaminar Co-kultur Primary Rat Kortikal neuroner og glia

DOI:
10.3791/3257-v

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12:32 min

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November 12, 2011

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, ,
1Department of Pharmacology and Physiology,Drexel University College of Medicine

Capítulos

  • 00:05Título
  • 01:18Glia Culture (2 Weeks Before Plating Neurons)
  • 05:17Secondary Glia Culture (48 Hours Before Plating Neurons)
  • 07:18Neuronal Dissection and Culture
  • 11:29Representative Results of Bilaminar Co-culture of Primary Rat Cortical Neurons
  • 12:13Conclusion

Summary

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Her giver vi en protokol til dyrkning rotte cortical neuroner i overværelse af en glial feeder lag. De dyrkede neuroner etablere polaritet og skabe synapser, og kan adskilles fra den glia til brug i forskellige applikationer, såsom elektrofysiologi, calcium imaging, celle overlevelse assays, immuncytokemi, og RNA / DNA / protein isolation.

Tags

Bilaminar Co-cultureRat Cortical NeuronsGlial Feeder LayerExperimental NeedsGlass SubstratePlastic Growth SubstrateThermanoxAstroglia-enriched Secondary CultureNeuronal GrowthSurvivalDifferentiationTrophic FactorsBrain EnvironmentNeuronal-glial InteractionsGlia Contamination

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