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Bilaminar Co-culture of Primary Rat Cortical Neurons and Glia
Capitoli
- 00:05Titolo
- 01:18Glia Culture (2 Weeks Before Plating Neurons)
- 05:17Secondary Glia Culture (48 Hours Before Plating Neurons)
- 07:18Neuronal Dissection and Culture
- 11:29Representative Results of Bilaminar Co-culture of Primary Rat Cortical Neurons
- 12:13Conclusion
Here we provide a protocol for culturing rat cortical neurons in the presence of a glial feeder layer. The cultured neurons establish polarity and create synapses, and can be separated from the glia for use in various applications, such as electrophysiology, calcium imaging, cell survival assays, immunocytochemistry, and RNA/DNA/protein isolation.
