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Bioengineering

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JoVE Journal Bioengineering
Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
 

Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering

Article DOI: 10.3791/3251-v • 10:19 min • September 19th, 2011
September 19th, 2011 •
1, 1, 1
1Department of Biomedical Engineering, Tufts University

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Resumo
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We describe the isolation of neonatal cardiomyocytes and the preparation of the cells for encapsulation in fibrin hydrogel constructs for tissue engineering. We describe methods for analyzing the tissue engineered myocardium after the culture period including active force generated upon electrical stimulation and cell viability and immunohistological staining.

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Encapsulation Cardiomyocytes Fibrin Hydrogel Cardiac Tissue Engineering Three Dimensional Hydrogel Culture Conditions Synthetic Polymers PEG-DA PLGA Naturally Occurring Proteins Collagen Hyaluronic Acid Fibrin Polymerize Mesh Wound Healing Processes Cell-degradable Autologous Temporary Scaffold Isolation Of Neonatal Cardiomyocytes Rat Pups Preparation Of Cells Encapsulation In Fibrin Hydrogel Constructs In Vitro Studies Cardiac Tissue Formation And Engineering Fibrinogen Thrombin Fibrinopeptides FpA And FpB
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