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成像白细胞粘附到血管内皮细胞在高腔内压力
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Imaging Leukocyte Adhesion to the Vascular Endothelium at High Intraluminal Pressure
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成像白细胞粘附到血管内皮细胞在高腔内压力

DOI:
10.3791/3221-v

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06:20 min

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August 23, 2011

•

, , ,
1Vascular Pharmacology Laboratory, Baker IDI Heart and Diabetes Institute,Monash University

Capítulos

  • 00:05Título
  • 01:11Mounting the Vessel
  • 02:13Pressurizing the Vessel
  • 04:19Perfusion with While Blood
  • 05:18Representative Leukocyte Adhesion Results
  • 06:01Conclusion

Summary

Tadução automática

Tadução automática

这是一个可视化白细胞粘附到内皮收获压力容器的方法。该技术使研究不同的腔内压力高达200毫米汞柱，从而模拟高血压的病理生理条件下剪切流动的血管粘连。

Tags

Leukocyte AdhesionVascular EndotheliumHigh Intraluminal PressureHypertensionEndothelial DysfunctionInflammationAtherosclerosisChronic Kidney DiseaseStrokeHeart FailureAdhesion CascadeRollingTetheringAdherenceTransmigrationAdhesion MoleculesVCAM-1ICAM-1E-selectinCytokinesChemokinesReactive Oxygen SpeciesStatic Adhesion AssaysCell-to-cell AdhesionCell Adhesion MoleculesIn Vitro MethodsPressure On EndotheliumMonocyte AdhesionInflammatory MarkersPhysiological Flow Conditions

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