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Imaging Leukocyte Adhesion to the Vascular Endothelium at High Intraluminal Pressure
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August 23, 2011
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This is a method to visualise leukocyte adhesion to the endothelium in harvested pressurised vessels. The technique enables studying vascular adhesion under shear flow with differing intraluminal pressures up to 200 mmHg thus mimic-ing the pathophysiological conditions of high blood pressure.
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Michell, D. L., Andrews, K. L., Woollard, K. J., Chin-Dusting, J. P. Imaging Leukocyte Adhesion to the Vascular Endothelium at High Intraluminal Pressure. J. Vis. Exp. (54), e3221, doi:10.3791/3221 (2011).
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