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闪光光解笼状化合物在嗅感觉神经元的纤毛
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Neurociência
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JoVE Journal Neurociência
Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons
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闪光光解笼状化合物在嗅感觉神经元的纤毛

DOI:
10.3791/3195-v

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11:35 min

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October 29, 2011

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, ,
1SISSA,International School for Advanced Studies, 2Istituto di Biofisica,Consiglio Nazionale delle Ricerche, 3SISSA Unit,Italian Institute of Technology

Capítulos

  • 00:05Título
  • 00:08Introduction
  • 01:35Instrumentation
  • 03:37Dissociation of Olfactory Sensory Neurons
  • 07:51Recording
  • 09:37Representative Results
  • 11:09Conclusion

Summary

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笼状化合物的光解允许快速和本地化的各种生理活性化合物的浓度增加生产。在这里，我们将展示如何获得光解笼中的cAMP结合或分离小鼠嗅感觉神经元的嗅觉传导的研究为笼钙膜片钳记录。

Tags

Flash PhotolysisCaged CompoundsOlfactory Sensory NeuronsPatch-clamp RecordingsPhotolysis Of Caged CompoundsOlfactory TransductionDissociated Mouse Olfactory Sensory NeuronsCiliaOdorant BindingReceptorsCAMPCyclic Nucleotide-gated (CNG) ChannelsCa EntryCa-activated Cl ChannelsMouse Olfactory EpitheliumFlash LampUltraviolet FlashesWhole-cell Voltage-clamp Configuration

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