É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
扩展人外周血γδT细胞使用Zoledronate
26,063 Views
•
13:08 min
•
September 09, 2011
DOI:
Summary
Automatically generated
扩大γδT细胞从外周血单个核细胞（PBMC）的一个方法来描述。外周血单核细胞衍生的γδT细胞受到刺激和扩大使用zoledronate和白细胞介素2（IL - 2）。 γδT细胞的大规模扩张，可应用于自体肿瘤细胞免疫疗法。
Explore More Videos
Human Peripheral Bloodγδ T CellsExpansionZoledronateStress-induced AntigensAnti-tumor ActivityAnti-infective ActivityVγ9Vδ2 T Cell ReceptorMajor Histocompatibility Complex-independentCytolytic FunctionsTh1-like Effector FunctionsCancer ImmunotherapyPhosphoantigens(E)-4-hydroxy-3-methyl-but-2-enyl Pyrophosphate (HMBPP)Isoprenoid BiosynthesisIsopentenyl Pyrophosphate (IPP)Mevalonate PathwayDysregulationTumor CellsAminobisphosphonatesPamidronateFarnesyl Pyrophosphate Synthase (FPPS)Intracellular Levels Of IPP
Read Article
Cite this Article
Kondo, M., Izumi, T., Fujieda, N., Kondo, A., Morishita, T., Matsushita, H., Kakimi, K. Expansion of Human Peripheral Blood γδ T Cells using Zoledronate. J. Vis. Exp. (55), e3182, doi:10.3791/3182 (2011).
Copy