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DNA Metilasyonu: bisülfit modifikasyonu ve Analiz
JoVE Journal
Biologia
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JoVE Journal Biologia
DNA Methylation: Bisulphite Modification and Analysis
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DNA Metilasyonu: bisülfit modifikasyonu ve Analiz

102,474 Views

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12:34 min

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October 21, 2011

DOI:

10.3791/3170-v

DOI:
10.3791/3170-v

•

12:34 min
October 21, 2011

•

48 Views
, , ,
1Epigenetics Group, Cancer Research Program,Garvan Institute of Medical Research, 2St Vincent’s Clinical School,University of NSW

Summary

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DNA metilasyon analizi için altın standart bisülfit dönüştürülen DNA genom dizileri. Bu yöntem, asidik koşullar altında deaminasyon bisülfit 5-methylcytosine (5-MEC) ile karşılaştırıldığında sitozin artan duyarlılık yararlanır. Unmethylated cytosines hedef genomik DNA PCR sonra metil cytosines ayırt edilebilir.

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Cite this Article

Patterson, K., Molloy, L., Qu, W., Clark, S. DNA Methylation: Bisulphite Modification and Analysis. J. Vis. Exp. (56), e3170, doi:10.3791/3170 (2011).

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