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DNA Methylation: Bisulphite Modification and Analysis
JoVE Revista
Biología
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JoVE Revista Biología
DNA Methylation: Bisulphite Modification and Analysis
DOI:

12:34 min

October 21, 2011

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Capítulos

  • 00:05Título
  • 01:52DNA Preparation and Denaturation
  • 02:57Bisulphite Deamination
  • 06:38PCR Amplification
  • 09:08Results of DNA Methylation Analysis
  • 12:15Conclusion

Summary

Traducción Automática

The gold standard for DNA methylation analysis is genomic sequencing of bisulphite converted DNA. This method takes advantage of the increased sensitivity of cytosine compared with 5-methylcytosine (5-MeC) to bisulphite deamination under acidic conditions. Unmethylated cytosines can be distinguished from methylated cytosines after PCR amplification of the target genomic DNA.

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