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FM染料を用いて培養小脳顆粒神経細胞のシナプス小胞プールの補充の定量分析

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes

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FM染料を用いて培養小脳顆粒神経細胞のシナプス小胞プールの補充の定量分析

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09:02 min

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November 11, 2011

DOI:

DOI:

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09:02 min

November 11, 2011

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3 Views

Giselle Cheung, Michael A. Cousin

¹Membrane Biology Group, Centre for integrative Physiology,University of Edinburgh

Tags

Quantitative Analysis Synaptic Vesicle Pool Replenishment Cultured Cerebellar Granule Neurons FM Dyes Neurotransmitter Release Endocytosis Recycling Pool Readily Releasable Pool Reserve Pool Assay SV Traffic Clathrin-dependent Endocytosis Activity-dependent Bulk Endocytosis Mobilisation Mechanisms SV Turnover Central Nerve Terminals Hydrophobic Hydrocarbon Tail Hydrophilic Head Group Fluorescent Probes

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