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JoVE Journal Neuroscience
Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling
 

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling

Article DOI: 10.3791/3125-v • 10:07 min • November 13th, 2011
November 13th, 2011 •
1, 1, 1, 1
1Center for Sleep and Circadian Biology, Northwestern University

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RNA expression profiling of discrete mouse brain regions requires a precise and repeatable tissue collection strategy. A protocol that uses both coronal brain sectioning and tissue corer-assisted microdissection is described here. The yield and quality of total RNA obtained from the resulting samples confirms the utility of the outlined method.

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Non-laser Capture Microscopy Microdissection Mouse Brain Regions Total RNA Isolation Next-generation Sequencing Gene Expression Profiling Technological Platforms Next-generation Sequencing Microarray QRT-PCR High-resolution RNA Sequencing RNA-Seq Tissue-specific Expression Levels Alternative Splicing Micro RNAs Whole Transcriptome Profiling Discrete Tissues Phenotypically Distinct Groups Molecular Mechanisms Physiological States Human Brain Tissue Disease Progression Next-generation Sequencing Integration Gene Expression Studies Mouse Models Brain Nuclei Precision Laser Capture Microscopy (LCM) Sample Excision
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